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低能N离子注入对蚕豆根尖细胞的诱变效应
引用本文:黄雅琴,李尽哲,黄群策. 低能N离子注入对蚕豆根尖细胞的诱变效应[J]. 原子核物理评论, 2012, 29(3): 290-293. DOI: 10.11804/NuclPhysRev.29.03.290
作者姓名:黄雅琴  李尽哲  黄群策
作者单位:1.信阳农业高等专科学校, 河南 信阳 464000; 2.郑州大学离子束生物工程省重点实验室, 河南 郑州 450052
基金项目:河南省科技前沿基础研究项目
摘    要:为了研究低能N离子束的细胞遗传学效应, 以不同剂量的N离子束对蚕豆种子的种胚进行辐照, 观察分析根尖细胞的微核率、 有丝分裂指数和染色体畸变效应。 研究发现, 离子束的注入抑制了根尖细胞的有丝分裂, 干扰了正常的有丝分裂过程, 引发了染色体的结构、 行为和数目畸变; 随着离子注入剂量的增加, 微核率增加、 有丝分裂指数降低、 染色体畸变率增加。 In order to study the cytogenetic effects of low energy nitrogen ion irradiation, broad bean seed embryo was irradiated by different doses of nitrogen ions. Micronucleus rate, mitotic index and chromosome aberration in root tip cells were analyzed. The results showed that the injection of ions inhibited mitosis of root tip cells, interfered the normal process of mitosis, caused aberrations of chromosome structure, behavior and number. The frequency of micronucleus and chromosomal aberrations increased with the increasing radiation dosage, while mitotic index decreased.

关 键 词:N离子束   蚕豆   根尖细胞   染色体畸变   微核
收稿时间:1900-01-01

Effects of Irradiation with Low-energy Nitrogen Ion Injection on Root Tip Cells of Broad Bean
HUANG Ya-qin,LI Jin-zhe,HUANG Qun-ce. Effects of Irradiation with Low-energy Nitrogen Ion Injection on Root Tip Cells of Broad Bean[J]. Nuclear Physics Review, 2012, 29(3): 290-293. DOI: 10.11804/NuclPhysRev.29.03.290
Authors:HUANG Ya-qin  LI Jin-zhe  HUANG Qun-ce
Affiliation:1.Department of Biotechnology, Xinyang Agricultural College, Xinyang 464000, Henan, China; 2.Henan Provincial Key Laboratory of Ion Beam Bio engineering, Zhengzhou University , Zhengzhou 450052, Henan, China
Abstract:In order to study the cytogenetic effects of low-energy nitrogen ion irradiation,broad bean seed embryo was irradiated by different doses of nitrogen ions.Micronucleus rate,mitotic index and chromosome aberration in root-tip cells were analyzed.The results showed that the injection of ions inhibited mitosis of root tip cells,interfered the normal process of mitosis,caused aberrations of chromosome structure,behavior and number.The frequency of micronucleus and chromosomal aberrations increased with the increasing radiation dosage,while mitotic index decreased.
Keywords:nitrogen ion beam  broad bean  root tip cell chromosomal aberration micronucleus
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