聚焦超声辐照在体兔肝的生物效应——组织化学变化

用强度为30W/cm²,频率为1.06MHz的连续聚焦超声直接辐照在体兔肝3min,辐照后分别于一天、三天、七天、一月和二月宰杀。把肝的照射区做成切片,包括ATPase、TPPase、NSEase、SDHase、糖元和一般结构的显示。观察中发现:（1）一些损伤的肝细胞内产生空化泡,使整个细胞膨大成球形,并压迫核移向细胞边缘,由于球形体相互之间接触面减小,因而各自游离;（2）重损伤区ATPase、TPPase、NSEase、SDHase的活性低落或消失,是细胞即将死亡的表现;（3）轻损伤区ATPase、NSEase、SDHase活性增强,认为是由于损伤刺激引起反馈的结果;（4）轻损伤区TPPase活性减弱,说明在此情况下,肝细胞仍有分泌胆汁的功能;（5）轻损伤区和重损伤区的肝糖元都消失。

BIOLOGICAL EFFECT OF THE RABBIT LIVER IRRADIATED WITH FOCUSED ULTRASOUND IN VIVO-HISTOCHEMICAL CHANGES

Rabbits’ livers were irradiated by focused ultrasound at the intensity of 30 W/cm²,frequency 1.06 MHz,for 3 mins. directly. The treated animals were sacrificed at 1 day,3 days,7 days,1 month and 2 months after irradiation. Then the irradiated area of the liver was cut off and studied with routine histological H. E. method and some histochemical techniques,including the demonstration of adenosine triphosphatase （ATPase）,thiamin pyrophosphatase （TPPase）,nonspecific esterase （NSEase）,succinate dehydrogenase （SDHase）,and glycogen. The histological and histochemical changes in this experiment are as follows:
（1） The cavitational vesicles which are produced in the cytoplasm of light lesion hepatic cell,squeeze the nucleus to margin of the cell,and extend the contour of hepatic cell to a ball-shape appearance. All the ball-shape cells in the hepatic cord decrease their contact surface and are apt to separate from one another.
（2） The activities of ATPase,TPPase,NSEase and SDHase in the heavy lesion cells decrease vigorously,even no activities can be seen. Such phenomenon repesents necrosis of these cells.
（3） The reactions of ATPase,NSEase and SDHase in the light lesion cell are stronger than that of normal cells. It seems to be the result of a feedback of lesion.
（4） The slight decrease of the activity of TPPase in the light lesion cells indicates that the cells retain the function of bile secretion.
（5） Glycogen granules disappear both in light and heavy lesion regions.