In vitro replication study of modified bases in ras sequences. |
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| Authors: | H Kamiya T Sakaguchi N Murata M Fujimuro H Miura H Ishikawa M Shimizu H Inoue S Nishimura A Matsukage |
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| Institution: | Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan. |
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| Abstract: | DNA templates containing a modified base (O6-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th or 61st codon of a ras gene were synthesized and deoxynucleotide incorporation opposite the lesions was investigated. The templates were replicated by Taq DNA polymerase, recombinant rat DNA polymerase beta and mouse DNA polymerase alpha-primase complex. Sequence analysis of the replicated products indicated selective incorporation of nucleotide(s) opposite a modified base, depending on the kind of base and of DNA polymerase. This system is very useful to obtain results of in vitro replication of modified bases in ras sequences. |
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