Detection of CD4~+ T-lymphocytes from hemodialyzed patients by surface plasmon resonance

A surface plasmon resonance (SPR) method was presented to discriminate hemodialyzed T-lymphocytes from the normal based on antibody-cell recognition. By dynamic reaction with fixed anti-human CD4 antibody, SPR could offer significant signals to distinguish hemodialyzed patients from the healthy controls within 200 sec after the cell injection in respect of either rising speed or maximum binding capacity (p＜0.01). The ratio method is also used to exclude the non-specific adsorption. The percentage of hemodialyzed patients' CD4⁺ T cells against the healthy control is 69 ± 18 %. The most attractive of the present method is its ability to detect the intact and label-free lymphocytes, and further to detect the subpopulations, or proteins secreted by the desired lymphocytes subset.