| 羧甲基牛膝多糖的制备、结构及生物活性研究 |
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| 引用本文： | 邓乐华,田庚元.羧甲基牛膝多糖的制备、结构及生物活性研究[J].化学学报,2002,60(11):2049-2055. |
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| 作者姓名： | 邓乐华 田庚元 |
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| 作者单位： | 中国科学院上海有机化学研究所.上海(200032) |
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| 摘 要： | 在NaOH水溶液中，以ClCH_2COOH为羧甲基化试剂，对牛膝多糖(AbPS)进行了羧 甲基化，粗产物经DEAE-Cellulose及Sephadex G-25两次柱层析纯化，得到羧甲基 牛膝多糖(CM-AbPS)纯品，经高效液相法（HPLC）及毛细管电泳法（CE）检测表明 具有较好的均一性，并通过样品甲基化和GC-MS分析，对羧甲基牛膝多糖链中羧甲 基的分布进行了研究，结果表明羧甲基牛膝多糖中羧甲基主要取代的糖链中呋喃果 糖的4-位上。该产物具有抗肿瘤活性。
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| 关 键 词： | 牛膝 多糖 羧甲基 高速液体色谱 抗肿瘤药 |
| 修稿时间： | 2002年4月29日 |
Studies on the Preparation, Structure and Bioactivity of CM-AbPS |
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| DENG,Le Hua TIAN,Geng Yuan.Studies on the Preparation, Structure and Bioactivity of CM-AbPS[J].Acta Chimica Sinica,2002,60(11):2049-2055. |
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| Authors: | DENG Le Hua TIAN Geng Yuan |
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| Institution: | State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences. Shanghai(200032) |
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| Abstract: | Achyranthes bidentata Polysaccharides (AbPS) was carboxymethylated by ClCH 2COOH in aqueous NaOH and the crude product was purified by successive DEAE Cellulose and Sephadex G 25 column chromatography to yield homogeneous carboxymethylated product (CM AbPS), which was detected by high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). By the methylation method and GC MS analysis, we studied the distribution of carboxymethyl groups in the sugar backbone of CM AbPS and conclude that the carboxymethyl groups mainly substitute on 4 position of the fructofuranose of the sugar backbone. The product has anti tumor activity. |
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| Keywords: | CM AbPS carboxymethylation GC MS analysis structural analysis anti tumor |
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