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Efficiency of fluorescence excitation in chlorophyll <Emphasis Type="Italic">a</Emphasis> by the second and third harmonics of emission from an Nd:YAG laser
Authors:O A Bukin  S S Golik  P A Salyuk  E N Baulo  I A Lastovskaya
Institution:(1) Institute of Automation and Control Processes, Far Eastern Branch of the Russian Academy of Sciences, 5 ul. Radio, Vladivostok, 690041, Russia;(2) V. I. Il’ichev Pacific Oceanology Institute, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia;(3) Far Eastern State Technical Fishery University, Vladivostok, Russia
Abstract:We have studied the efficiency of fluorescence excitation in chlorophyll a by the second harmonic (532 nm) and the third harmonic (355 nm) of an Nd:YAG laser for sea water samples with phytoplankton taken during different algal blooms and for extracts of higher plants diluted with sea water. We have shown that when using the second harmonic, the efficiency of fluorescence excitation in chlorophyll a is more than twice as high as for excitation by the third harmonic. We discuss the advisability of using the second or third harmonics of the emission from an Nd:YAG laser when studying the biooptical parameters of sea water. __________ Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 75, No. 2, pp. 224–227, March–April, 2008.
Keywords:fluorescence  chlorophyll a  phytoplankton  Nd:YAG laser
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