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| 非标记型河虾免疫传感器的制备及应用 | |
| 引用本文： | 汪忠云,方银军,孙秀兰,李在均,陈佩佩. 非标记型河虾免疫传感器的制备及应用[J]. 分析试验室, 2009, 28(11) |
| 作者姓名： | 汪忠云 方银军 孙秀兰 李在均 陈佩佩 |
| 作者单位： | 1. 江南大学化学与材料工程学院,无锡,214122 2. 浙江赞宇科技股份有限公司,杭州,310009 3. 江南大学食品学院,无锡,214122 |
| 基金项目： | 国家自然科学基金，国家高技术计划"863" |
| 摘 要： | 四乙氧基硅在HCl催化下水解形成硅溶胶。将硅溶胶与河虾抗体混合均匀后,涂于玻碳电极表面制备得非标记型河虾抗体免疫传感器。采用Fe(CN)63-/4-的磷酸盐缓冲溶液(pH 6.5)为测试底液,研究此传感器在免疫反应中的循环伏安和交流阻抗特性。结果显示免疫反应后传感器的循环伏安图上未出现新的氧化-还原峰,表明该免疫反应属于非氧化还原过程。采用交流阻抗监测电极表面,发现传感器阻抗值随虾过敏原浓度的增加而增大。对交流阻抗图进行分析,结果表明电极表面的免疫反应是一个受电子转移控制的过程。当虾过敏原浓度在0~10 ng/mL之间,电极表面电子转移阻抗的增加值与虾过敏原浓度呈线性关系,检出限为0.1 ng/mL(S/N=3)。该方法已成功应用于河虾制品中过敏原的测定。 |
| 关 键 词： | 虾过敏原 非标记 免疫传感器 交流阻抗 |
Preparation of a non-labeled river shrimp antibody immunosensor and its application |
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| WANG Zhong-yun,FANG Yin-jun,SUN Xiu-lan,LI Zai-jun,CHEN Pei-pei. Preparation of a non-labeled river shrimp antibody immunosensor and its application[J]. Chinese Journal of Analysis Laboratory, 2009, 28(11) | |
| Authors: | WANG Zhong-yun FANG Yin-jun SUN Xiu-lan LI Zai-jun CHEN Pei-pei |
| Affiliation: | WANG Zhong-yun1,FANG Yin-jun3,SUN Xiu-lan2,LI Zai-jun1 and CHEN Pei-pei1(1.School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,2/ School of Food Science and Technology,3.Zhejiang Zanyu Technology Co.,LTD.,Hangzhou 310009) |
| Abstract: | Tetraethoxysilicane was hydrolyzed into silica sol in the presence of hydrochloric acid.After that,a non-labeled immunosensor was fabricated by dripping the mixture of the silica sol ane river shrimp antibody on the surface of the glassy carbon electrode.In this study,cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS) were employed to investigate the electrochemical performance of immunoreaction of river shrimp allergen with the antibody,in which the sensor and Fe(CN)63-/4-phosphate buff... |
| Keywords: | Allergen River shrimp Non-labeled immunosensor Electrochemical impedance spectroscopy |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |