Hydrogen peroxide biosensor with a supramolecular layer-by-layer design |
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Authors: | Camacho Conrado Matías Juan C Cao Roberto Matos Madyu Chico Belkis Hernández Javier Longo Maria A Sanromán Maria A Villalonga Reynaldo |
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Affiliation: | Center for Enzyme Technology, University of Matanzas, Matanzas 44740, Cuba. |
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Abstract: | A new sensor design is reported for the construction of an amperometric enzyme biosensor toward H (2)O(2). It was based in the supramolecular immobilization of alternating layers of horseradish peroxidase (either modified with 1-adamantane or beta-cyclodextrin-branched carboxymethylcellulose residues) on Au electrodes coated with polythiolated beta-cyclodextrin polymer. The analytical response of the electrodes, using 1 mM hydroquinone as an electrochemical mediator, increases when the number of enzyme layers increases. The biosensor having three enzyme layers showed a sensitivity of 720 microA/M cm (2) and a detection limit of 2 microM and retained 96% of its initial activity after 30 days of storage. The host-guest supramolecular nature of the immobilization method was confirmed by cyclic voltammetry. |
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