Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and proteins |
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Authors: | Cheng-Kang Chiang Mi-Chen Chiang Zong-Hong Lin Guo-Yu Lan Yang-Wei Lin Huan-Tsung Chang |
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Institution: | 1.Department of Chemistry,National Taiwan University,Taipei,Taiwan |
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Abstract: | We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using
surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomateriais
for small analytes (e.g., glutathione); Pt nanosponges and Fe3O4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomateriais have several
advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations
(fewer problems associated with “sweet spois”), when used in laser desorption/ionization mass spectrometry. |
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Keywords: | |
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