A novel strategy for MALDI-TOF MS analysis of small molecules |
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Authors: | Shu Zhang Jian’an Liu Yi Chen Shaoxiang Xiong Guanghui Wang Jun Chen Guoqiang Yang |
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Institution: | 1. Institute of Chemistry, Chinese Academy of Sciences, 100190, Beijing, China 2. Beijing National Laboratory for Molecular Science, 100190, Beijing, China
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Abstract: | Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) does not work efficiently on small
molecules (usually with molecular weight below 500 Da) because of the interference of matrix-related peaks in low m/z region. The previous methods developed for this problem focused on reducing the peaks caused by the traditional matrices.
Here, we report a novel strategy to analyze small molecules in a high and interference-free mass range by using metal-phthalocyanines
(MPcs) as matrices which should be capable of forming matrix-analyte adducts. The mass of the target analyte was calculated
by subtracting the mass of MPc from the mass of the MPc-analyte adduct. MPcs were also detectable and could serve as internal
standards. Various MPcs with aromatic or aliphatic groups and different metal centers were then synthesized and explored.
Aluminum-phthalocyanines (AlPcs), gallium-phthalocyanines (GaPcs), and indium-phthalocyanines (InPcs) were efficient matrices
to form MPc-analyte adducts in either the positive or negative ion mode. The detection limits varied from 17 to 75 fmol, depending
on analyte types. The mechanism of adducts formation was also proposed. Collectively, our strategy provides a novel and efficient
way to analyze small molecules by MALDI-TOF MS. |
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