Analysis of oligomannuronic acids and oligoguluronic acids by high-performance anion-exchange chromatography and electrospray ionization mass spectrometry |
| |
| Authors: | Liu Y Jiang X L Cui H Guan H S |
| |
| Institution: | Food Science and Technology, Fishery College, Ocean University of Qingdao, China. |
| |
| Abstract: | Oligomannuronic acids and oligoguluronic acids were prepared by enzymatic hydrolysis of alginate with alginate lyases. The oligosaccharides generated up to degree of polymerization 16 were characterized by high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) and electrospray ionization mass spectrometry (ESI-MS). Acetate buffer linear gradients were used as mobile phases for separations of oligosaccharides. ESI-MS and HPAEC-PAD are very effective for the analysis and characterization of anionic oligosaccharides. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
