野生藜蒿中的微量元素的质谱分析

研究应用电感耦合等离子体串联质谱(ICP-MS/MS)技术建立准确测定野生藜蒿中12个微量元素的分析方法。采用MS/MS模式，以O₂为反应气，元素As和Se与O₂发生质量转移反应生成AsO和SeO，通过测定AsO和SeO消除质谱干扰；以NH₃/He为反应气，元素Cr，Mn，Fe，Co，Ni，Cu，Zn与NH₃/He发生质量转移反应形成团簇离子，通过测定团簇离子消除质谱干扰。元素Cd，Hg和Pb采用标准模式进行测定。12个待测元素的校准曲线在0～200 μg·L-1范围内具有良好的线性关系，各元素的检出限为0.64～49.61 ng·L-1。通过测定国家标准物质芹菜(GBW10048)，采用t检验法进行差异性检验表明，测定值与标准值之间无显著性差异，验证了方法的准确可靠性。各元素的加标回收率在92.0%～106.1%之间，相对标准偏差(RSD)为1.6%～4.9%。分析了来自国内不同地区的4个野生藜蒿样品，结果显示，不同来源样品中12个元素的含量各有差异，Mn，Fe，Zn的含量远高于其余九种元素，毒理性元素As，Cd，Hg和Pb的含量处于极低水平。方法能准确测定野生藜蒿中的多种微量元素，为野生藜蒿的食用营养和安全提供科学的理论依据。

Analysis of Trace Elements in Wild Artemisia Selengensis Using Inductively Coupled Plasma Tandem Mass Spectrometry

An analytical method for the accurate determination of 12 kinds of trace elements in wild Artemisia Selengensis by inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) was established. In the MS/MS mode, AsO and SeO were formed by mass shift reaction of As and Se with O₂, and AsO and SeO were used to eliminate mass spectral interferences. Using NH₃/He as the reaction gas, Cr, Mn, Fe, Co, Ni, Cu, and Zn were reacted with NH₃/He to form clusters ions, by measuring the cluster ions to eliminate mass spectral interferences. Cd, Hg and Pb were measured using standard mode. The calibration curves of analytes in the 0～200 μg·L-1 range has a good linear relationship, and the detection limit is 0.64～49.61 ng·L-1. By analyzing the national standard material celery (GBW 10048), the difference test was carried out by t test, and the results showed that there was no significant difference between the measured value and the certified value, and the method was proved to be accurate and reliable. The spike recovery was 92.0%～106.1%, and the RSD was 1.6%～4.9% for twelve elements. Analyzing from 4 samples of wild Artemisia Selengensis in different areas of China, according to the results, different sources of 12 elements content in the samples vary, the contents of Mn, Fe, Zn are much higher than the rest of the 9 kinds of elements, and poison rational elements of As, Cd, Hg, Pb content are very low. The method can accurately determine a variety of trace elements in the wild Artemisia Selengensis, and provide scientific theoretical basis for the edible nutrition and safety of the wild Artemisia Selengensis.