叶绿素荧光技术在植物表型分析的研究进展

作物优良品种选育是实现作物优质高产的关键。现代育种方法需要获取植株的大量表型信息，最终选育出性状稳定的优良品种。近年来，高通量植物表型分析技术因其快速、无损、高效等优势，为筛选优良作物品种提供了技术保障，已成为农学、工程、计算机科学等多学科交叉研究的热点。其中，叶绿素荧光技术作为植物光合作用的探针，是研究植物逆境胁迫表型的有力工具之一，能够实现植物生物与非生物胁迫的高效分析，加快作物优良性状的筛选。该文旨在阐述叶绿素荧光技术的研究进展和发展趋势，主要介绍了叶绿素荧光技术的基本原理和成像系统、叶绿素荧光参数的分析和处理方法，总结了在植物表型分析研究中的应用情况，探讨了该技术目前存在的问题和改进的方法，进一步展望了叶绿素荧光技术在植物表型分析中的应用前景。

Applications of Chlorophyll Fluorescence in Plant Phenotyping: A Review

Plant breeding is important for increasing the yield and improving the quality of crop. Modern techniques of plant breeding require various phenotypic traits obtained from a large number of crops in order to cultivate varieties with useful traits. Researchers have been interested in developing nondestructive, fast and efficient plant phenotyping techniques, which could bridge the knowledge gap between the plant phenotype and the genotype. The chlorophyll fluorescence imaging can provide information related to the plant photosynthesis as one of the useful tools in the current phenotyping techniques, which can be used in plant responses to biotic and abiotic stresses and promote the selection of useful plant traits. This paper presents a brief introduction of fluorescence imaging system, methods of fluorescence image analysis and applications in plant phenotyping, and demonstrates challenge issues, technical solutions and potential applications of chlorophyll fluorescence imaging in plant phenotyping.