| LIMITED TRYPTIC DIGESTION OF LEUCYL-tRNA SYNTHETASE AND CHARACTERIZATION OF ITS ACTIVE FRAGMENT |
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| 引用本文： | 缪枫,施建平,王应睐. LIMITED TRYPTIC DIGESTION OF LEUCYL-tRNA SYNTHETASE AND CHARACTERIZATION OF ITS ACTIVE FRAGMENT[J]. 中国科学B辑(英文版), 1989, 0(5) |
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| 作者姓名： | 缪枫 施建平 王应睐 |
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| 作者单位： | Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica |
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| 基金项目： | Project supported by the National Natural Science Foundation of China. Part of the results was presented at the 4th Congress of FAOB (Federation of Asian and Oceanian Biochemists) in 1986. |
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| 摘 要： | Leucyl-tRNA synthetase (LeuRS, EC 6.1.1.4) from E. coli underwent limitedproteolysis by trypsin which cut off 6K peptide and converted the intact LeuRS into a 96K fragment. The truncated enzyme retained the PPi exchange activity with the same kinetic parameters as those of native LeuRS but lost the tRNA~(Leu)charging, binding and other tRNA~(Leu)-related activities. N-terminus analysis showed that the 6K peptide was located at the C-terminus of LeuRS. This small part played a crucial role in tRNA~(Leu) binding. Our results suggest that the two activities, PPi exchange and tRNA charging are independent of each other and correspond to different structural regions of LeuRS. The C-terminal region might be the tRNA~(Leu)binding site of LeuRS.
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LIMITED TRYPTIC DIGESTION OF LEUCYL-tRNA SYNTHETASE AND CHARACTERIZATION OF ITS ACTIVE FRAGMENT |
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| MIAO FENG SHI JIAN-PING WANG YING-LAI. LIMITED TRYPTIC DIGESTION OF LEUCYL-tRNA SYNTHETASE AND CHARACTERIZATION OF ITS ACTIVE FRAGMENT[J]. Science in China(Chemistry), 1989, 0(5) |
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| Authors: | MIAO FENG SHI JIAN-PING WANG YING-LAI |
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| Abstract: | Leucyl-tRNA synthetase (LeuRS, EC 6.1.1.4) from E. coli underwent limitedproteolysis by trypsin which cut off 6K peptide and converted the intact LeuRS into a 96K fragment. The truncated enzyme retained the PPi exchange activity with the same kinetic parameters as those of native LeuRS but lost the tRNA~(Leu)charging, binding and other tRNA~(Leu)-related activities. N-terminus analysis showed that the 6K peptide was located at the C-terminus of LeuRS. This small part played a crucial role in tRNA~(Leu) binding. Our results suggest that the two activities, PPi exchange and tRNA charging are independent of each other and correspond to different structural regions of LeuRS. The C-terminal region might be the tRNA~(Leu)binding site of LeuRS. |
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| Keywords: | synthetase limited tryptic digestion active fragment. |
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