Advantages of deuterium modification of nitroxide spin labels for biological epr studies |
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| Authors: | Albert H Beth Ray C Perkins Sindhaghatta D Venkataramu Donald E Pearson Charles R Park Jane H Park Larry R Dalton |
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| Institution: | Departments of Physiology and Chemistry, Vanderbilt University Nashville. Tennessee 37232, USA;Department of Chemistry, State University of New York at Stony Brook, Long Island, New York 11794, USA |
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| Abstract: | The spin label, perdeuterio-N-(1-oxy 1-2,2,6,6-tetramenthyl-4-piperidinyl)maleimide (DMSL) was synthesized and its EPR and saturation transfer EPR spectra were compared to those of the hydrogen analogue, HMSL- The labels were studied as freely tumbling entities and also bound to bovine serum albumin (BSA). Significant gains in spectral resolution and detectability were observed for DMSL relative to HMSL. |
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| Keywords: | Permanent address: Department of Inorganic Chemistry Hebrew University Jerusalem Israel |
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