The influence of molecular weight of dextran forming a heparin-bonding polysaccharide layer on the chromatographic properties of sorbents for HPAC analysis of human antithrombin III

Summary Human antithrombin III (AT III) is a natural anticoagulant of blood. It is separated from blood preparations by bioselective sorption on sorbents containing heparin as a complementary ligand interacting with AT III. The paper deals with the behaviour of the chromatographic packings obtained by bonding heparin to cross-linked polysaccharide layers deposited on controlled-porosity glass. The properties of the prepared sorbents are discussed with respect to molecular weight of dextran used to form the polysaccharide layers. The results obtained show that when ligand (heparin) and AT III are used the molecular weight of cross-linked dextrans does not significantly influence the chromatographic properties of the affinity packings.