Double fluorescent labeling method used for a study on liposomes |
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| Authors: | H Sawahara S Goto N Kinoshita |
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| Affiliation: | Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan. |
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| Abstract: | Carboxyfluorescein (CF) and Calcein (CAL) have almost the same fluorescent spectrum. However, only CAL forms a nonfluorescent chelate with the Co2+ ion. It was proven that this phenomenon can be used for the fractional determination of CF and CAL. And this fractional determination method was available for a study on liposomes. |
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