Bioconversion of proposed precursors into theobroxide and related compounds |
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Authors: | Li Peng Takahashi Kosaku Elkhateeb Ahmed Matsuura Hideyuki Yoshihara Teruhiko Nabeta Kensuke |
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Affiliation: | Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita ku, Sapporo 060-8589, Japan. yolipeng@criepi.denken.or.jp |
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Abstract: | We have previously reported a tetraketide origin for theobroxide and its related compound. In the present study, bioconversion of natural and deuterium-labeled precursors of this proposed biosynthetic pathway by Lasiodipoldia theobromae was investigated. Theobroxide was quantified after bioconversion from each proposed precursor. The transformation of the isotopically labeled precursor to products was tracked by 2H NMR measurement. |
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