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In situ electrodeposited nanoparticles for facilitating electron transfer across self-assembled monolayers in biosensor design
Authors:Du Dan  Ding Jiawang  Cai Jie  Zhang Jianming  Liu Li
Institution:

aKey Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China

bThe Technology Center of Wuhan Iron & Steel Company, Wuhan 430080, PR China

Abstract:Gold nanoparticles (AuNPs) were synthesized in situ and electrodeposited onto Au substrate. The AuNPs modified interface facilitates electron transfer across self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA). After activation of surface carboxyl groups with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, the interface displayed good stability for immobilization of biomolecules. These modification processes were characterized by contact angle measurement, cyclic voltammetry and electrochemical impedance spectra. The immobilized acetylcholinesterase (AChE), as a model, showed excellent activity to its substrate, leading to a stable AChE biosensor. Under the optimal experimental conditions, the inhibition of malathion on AChE biosensor was proportional to its concentration in two ranges, from 0.001 to 0.1 μg mL?1 and from 0.1 to 25 μg mL?1, with detection limit of 0.001 μg mL?1. The simple method showed good reproducibility and acceptable stability, which had potential application in biosensor design.
Keywords:Electrodeposition  Gold nanoparticles  Self-assembled monolayers  Organophosphate pesticides  Biosensor
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