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In situ electrodeposited nanoparticles for facilitating electron transfer across self-assembled monolayers in biosensor design
Authors:Du Dan  Ding Jiawang  Cai Jie  Zhang Jianming  Liu Li
Institution:

aKey Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China

bThe Technology Center of Wuhan Iron & Steel Company, Wuhan 430080, PR China

Abstract:Gold nanoparticles (AuNPs) were synthesized in situ and electrodeposited onto Au substrate. The AuNPs modified interface facilitates electron transfer across self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA). After activation of surface carboxyl groups with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, the interface displayed good stability for immobilization of biomolecules. These modification processes were characterized by contact angle measurement, cyclic voltammetry and electrochemical impedance spectra. The immobilized acetylcholinesterase (AChE), as a model, showed excellent activity to its substrate, leading to a stable AChE biosensor. Under the optimal experimental conditions, the inhibition of malathion on AChE biosensor was proportional to its concentration in two ranges, from 0.001 to 0.1 μg mL−1 and from 0.1 to 25 μg mL−1, with detection limit of 0.001 μg mL−1. The simple method showed good reproducibility and acceptable stability, which had potential application in biosensor design.
Keywords:Electrodeposition  Gold nanoparticles  Self-assembled monolayers  Organophosphate pesticides  Biosensor
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