Nucleic acid detection using carbon nanoparticles as a fluorescent sensing platform |
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Authors: | Li Hailong Zhang Yingwei Wang Lei Tian Jingqi Sun Xuping |
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Institution: | State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 Jilin, China. |
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Abstract: | In this communication, we demonstrate for the first time the proof of concept that carbon nanoparticles (CNPs) can be used as an effective fluorescent sensing platform for nucleic acid detection with selectivity down to single-base mismatch. The dye-labeled single-stranded DNA (ssDNA) probe is adsorbed onto the surface of the CNP via π-π interaction, quenching the dye. In the target assay, a double-stranded DNA (dsDNA) hybrid forms, recovering dye fluorescence. |
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