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Coating of titanium implant materials with thin polymeric films for binding the signaling protein BMP2 |
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| Authors: | Lorenz Corinna Hoffmann Andrea Gross Gerhard Windhagen Henning Dellinger Philip Möhwald Kai Dempwolf Wibke Menzel Henning |
| Institution: | 1. Institute for Technical Chemistry, Braunschweig University of Technology, Hans‐Sommer‐Stra?e 10, 38106 Braunschweig, Germany;2. Department of Gene Regulation and Differentiation, Helmholtz Center for Infection Research, Inhoffenstra?e 7, 38124 Braunschweig, Germany;3. Current address: Department of Trauma Surgery, Medical School Hannover, Carl‐Neuberg‐Str. 1, 30625 Hannover, Germany;4. Clinic for Orthopaedic Surgery in the Annastift Hospital, Medical School Hannover, Anna‐von‐Borries‐Str. 3, 30625 Hannover, Germany;5. Institute for Materials Science, Leibniz University Hannover, An der Universit?t 2, 30823 Garbsen, Germany |
| Abstract: | A fast and simple approach for immobilization using copolymers as interlayers is reported. The synthesized copolymers form stable self-assembled layers on implant materials like, e.g., titanium in a simple coating/drying/washing sequence and have functional groups which can bind proteins from an aqueous solution. The copolymer films have been characterized via ellipsometry and contact angle measurements and were tested for biocompatibility. An immunoassay was used to determine the amount of BMP2 and demonstrated an approximately 10-fold increase as compared to previously used self-assembled monolayers. A BMP2-responsive cell line with luciferase detection was used to determine the biological activity of the bound signaling protein. |
| Keywords: | biological application of polymers copolymerization implant modification proteins |
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