多维核磁共振波谱学

核磁共振波谱学自本世纪40年代中期诞生起,已走完了其光辉的50年历程,其间,它已完成了自连续波检测向脉冲傅里叶变换检测的过渡,并在此基础上率先将传统的一维谱的观测拓宽为多维谱的观测,不断提供了其它方法难以提供或无法提供的崭新信息,从而确立了它在分子结构和动力学研究中的独特地位,本文旨在以二维NMR为主,简述被称为现代核磁共振波谱学之核心的多维NMK的概况。

MULTIDIMENSIONAL NUCLEAR MAGNETECRESONANCE SPECTROSCOPY

ollowing the 50-year brilliant cvolution of NMR techniques, the outlines ofmuitidimensional NMR, especially 2D NMR-the core of modern NMR spectrosocpy,are given in this nunireview article. The text falls into three sections. After a briefintroduction of the Pulsc Fourier Tramform NMR, the fundainental mechanisms andexperimental categories, as well as the extensive applications of 2D NMR are intro-duced in soine detail in the next section. The insights into the design and the usageof the most useful 2D NMR pulse sequences, induding COSY types, NOESY(ROESY),heteronuclear chemical shift correlation experiments are described with application ex-aniples adopted from our laboratory. Multiple quantum coherences, such asINADEQUATE and HMBC/HMQC are mentioned as well. Finally, foundational ex-perimental methods and categories and enchanting perspectives of 3D/4D NMR in thefields of chemistry, biology and medicine are summarized in the third section.