Fluorescence chemosensors with pyrene and their interaction with nucleotide phosphate

A group of fluorescence chemosensor with pyrene, compounds (I), (II) and (III), were synthesized. The fluorescence spectra and the lifetime of these compounds were carefully measured. The fluorescence quenching spectra of pyrenyl butyric acid, compounds (I), (II) and (III) by different nucleotide phosphates, AMP ADP, ATP, dTTP, were also recorded and studied. The quenching and the stability constants were calculated by Stern-Volmer equation and eq. (2), respectively. The mechanism of interaction between fluorescence chemosensor and nucleotide phosphate was didscussed based on the comparison of the results obtained with the CPK model of free molecules of these com-pounds in the ground state. Project supported by the National Natural Science Foundation of China (Grant No. 29733100).