一种新型低噪音碳纤维纳米电极

近年来，碳纤维超微电极在生命科学领域中已取得了广泛应用，电极的超微尺寸使之能对生物微环境进行实时监测^1]，还可作为微柱分离的检测 ^2]，自Adams研究组1976年开展微电极伏安法对细胞外液中生物胺以及有关代谢物的检测研究以来，碳纤维超微电极已成为探测脑内甚至单个细胞内神经递质的一种有力的工具，人们已对单个细胞内神经递质^3]及激素^4]的释放进行了探索性研究。

A New Type of Low Noise Carbon Fiber Nanoelectrodes

A new and facile method has been developed for the fabrication of low noise carbon fiber nanoelectrodes. The carbon fiber was flame fused sealed in a tip of the glass capillary. The carbon fiber microelectrodes were made by cutting the protruding carbon fiber to a desired length, and the carbon fiber nanoelectrodes were achieved by etching the protruding carbon on the flame to form a nanometer scale tip. The tip of carbon fiber nanoelectrodes can be controlled within the range from 100 to 300 nm. Thus no epoxy wax was involved in the carbon fiber sealed in glass capillary procedure. The carbon fiber nanoelectrodes were inspected by scanning electron microscopy. The results demonstrated that the surface of the electrodes and the glass/fiber interface are very smooth. Thus the noise caused by the glass/fiber is much lower than that caused by fabricates conventionally. The electrodes were characterized by CV and FSCV measurements of the ferricyanide and 5 Hydroxytryptamine(5 HT) neurotransmitters. The results show that the carbon fiber nanoelectrodes have a very excellent electrochemical behaviors and high sensitivity. This experiments offers a new and facile method for the fabrication of carbon fiber nanoelectrodes with very high sensitivity and low noise.