Mercury arc lamp based super-resolution imaging with conventional fluorescence microscopes |
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| Institution: | 1. Institute of Organic Chemistry, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany;2. Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jia Tong University, 800 Dongchuan Road, Shanghai 200240, PR China;3. Swiss Tropical and Public Health Institute, Socinstrasse 57, CH-4002 Basel, Switzerland;4. University of Basel, Petersplatz 1, CH-4003 Basel, Switzerland;5. Institute of Pharmacy and Biochemistry, Department of Pharmaceutical Biology, University of Mainz, Staudinger Weg 5, D-55128 Mainz, Germany |
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| Abstract: | We present an implementation of localization based three-dimensional super-resolution imaging on a regular microscope. We retain the original arc lamp as the photoactivation light source, and incorporate an inexpensive diode laser for imaging. As alterations to the standard microscope is minimal, this optical setup can be easily adapted in a typical research laboratory and even undergraduate teaching experiments, providing an inexpensive system for students and research scientists who require such super resolution capabilities. With this simple design, a spatial resolution of better than 40 nm at a reasonable frame rate has been achieved, adequate for most routine applications. |
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| Keywords: | Mercury arc lamp Photoactivation source Localization-based super-resolution microscopy |
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