Microanatomy and ultrastructure of outer mantle epidermis of the cuttlefish,Sepia esculenta (Cephalopoda: Sepiidae) |
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| Affiliation: | 1. Department of Sea Cucumber Research, Jeollanamdo Ocean and Fisheries Science Institute, Jindo 539-802, Republic of Korea;2. Southwest Sea Fisheries Research Institute, NFRDI, Yeosu 556-823, Republic of Korea;3. Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea;1. Dept of Endocrinology, Xiang-Ya Hospital, Central South University, Changsha, China;2. Dept of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha, China;1. EA 6309 – Schools of Medicine and Pharmacy – University of Limoges, France;2. Service de Neurologie, Centre de référence national “neuropathies périphériques rares” – CHU Limoges, 87042 Limoges Cedex, France;1. Department of Anatomy, School of Medicine, Chung Shan Medical University, Taichung, Taiwan;2. Department of Medical Education, Chung Shan Medical University Hospital, Taichung, Taiwan;3. Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;4. Department of Anatomy, College of Medicine, China Medical University, Taichung, Taiwan;5. Department of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei, Taiwan;6. Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan;1. Experimental Neurology Unit and Milan Center for Neuroscience, University of Milano-Bicocca, 20900 Monza, Italy;2. Johns Hopkins Drug Discovery, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA;3. Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA;4. Department of Neuromuscular Medicine and Pathology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA;5. PhD Program in Neuroscience, University of Milano-Bicocca, 20900 Monza, Italy;6. PhD Program DIMET, University of Milano-Bicocca, 20900 Monza, Italy;7. Medpace, Cincinnati, OH 45227, USA;8. Helsinn Healthcare SA, 6915 Lugano, Switzerland;9. Department of Neuroscience, Medicine, Psychiatry and Oncology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA |
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| Abstract: | This study describes the ultrastructural characteristics of external epidermis of mantle of Sepia esculenta using light and electron microscopy. The epidermis was thicker on the ventral surface than on the dorsal surface, with a higher secretory cell distribution on the ventral surface than on the dorsal surface. The epidermis was a single layer composed of epithelial cells, secretory cells, ciliated cells and neuroglial cells. Epithelial cells were columnar with well-developed microvilli on the free surface, and the microvilli were covered with glycocalyx. The epithelial cells were connected to the neighboring cells by tight junctions and membrane interdigitations of the apico-frontal surface. Well-developed microfilaments were arranged in a vertical direction in the cortical cytoplasm. The secretory cells were categorized into three types (A, B and C) in accordance with the light microscopical characteristics and ultrastructures of the secretory granules. The distribution of these cells was in the following order: Type A > Type B > Type C. SEM observation revealed that the secretory pore size of the Type A secretory cells was approximately 8.6 μm × 12.2 μm. Cytoplasm displayed a red color as the result of Masson's trichrome stain and H–E stain, and contained polygonal granules of approximately 1.2 μm2 with a high electron density. The secretory pore size of the Type B secretory cells was approximately 10.1 μm × 12.1 μm. As the results of AB–PAS (pH 2.5) and AF–AB (pH 2.5) reactions, the cytoplasm displayed a red color. The cells contained membrane bounded secretory granules with very low electron density. The secretory pore of the Type C secretory cells was circular shape, and approximately 5.5 μm × 5.5 μm. Cytoplasm was found to be homogeneous under H–E stain and Masson's trichrome stain, and displayed a red color. As the result of AB–PAS (pH 2.5) reaction, the cytoplasm displayed a red color. The electron density of the secretory substance was the highest among the three types of secretory cells. The ciliated cells had a ciliary tuft on the free surface and were distributed throughout the mantle with the exception of the adhesive organs. Neuroglial cells were connected to the basal membrane, epithelial cells, secretory cells and nerve fibers through cytoplasmic process, and contained neurosecretory granules with high electron density within the cytoplasm. |
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| Keywords: | Mantle epidermis Ultrastructure |
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