Y2O3:Tm,Yb nanophosphors for correlative upconversion luminescence and cathodoluminescence imaging |
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| Institution: | 1. Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan;2. School of Engineering, The University of Shiga Prefecture, 2500 Hassaka, Hikone, Shiga 522-8533, Japan;1. Western Superconducting Technologies Co. Ltd., Xi’an 710018, China;2. Xi’an Technological University Institute of Information Engineering, 710025, China;3. School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China;1. School of Physics, Nankai University, Tianjin, 300071, China;2. The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Nankai University, Tianjin, 300457, China;1. School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150080, China;2. Department of Chemistry, Harbin Institute of Technology, Harbin 150001, China;1. Université de Sfax, Laboratoire de Physique Appliquée, Groupe des Matériaux Luminescents, Faculté des Sciences de Sfax, Département de Physique, Route de Soukra, Km 3.5, B.P. 1171 3000 Sfax, Tunisia;2. Department of Physics, University of Puerto Rico, San Juan, PR 00936, USA;3. School of Electrical Engineering and Computer Science, Ohio University, Athens, OH 45701, USA;1. Key Laboratory of Physics and Technology for Advanced Batteries (Ministry of Education), College of Physics, Jilin University, Changchun 130012, China;2. State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China |
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| Abstract: | We present a phosphor nanoparticle that shows both upconversion luminescence (UCL) and cathodoluminescence (CL). With this particle, low-autofluorescence, deep-tissue and wide-field fluorescence imaging can be achieved with nanometer-order high-spatial-resolution imaging. We synthesized Y2O3:Tm,Yb nanophosphors that emit visible and near-infrared UCL under 980 nm irradiation and blue CL via electron beam excitation. The phosphors were applied to fluorescent imaging of HeLa cells. The photostability of the phosphors was superior to that of a conventional organic dye. We show that after uptake by HeLa cells, the particles can be imaged with SEM and CL contrast in a cellular section. This indicates that correlative UCL and CL imaging of biological samples could be realized. |
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| Keywords: | Bioimaging Nanophosphor Cathodoluminescence Upconversion Correlative microscopy |
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