Micromorphology of cactus-pear (Opuntia ficus-indica (L.) Mill) cladodes based on scanning microscopies |
| |
| Affiliation: | 1. Laboratory of Plant Molecular Physiology, Centre of Biotechnology Borj-Cédria, P.B. 901, Hammam-Lif 2050, Tunisia;2. FAO-ICARDA CACTUSNET, 1, rue des Oliviers, El Menzah V., 2037 Tunis, Tunisia;1. División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Las Campanas, C.P. 76010 Santiago de Querétaro, Qro., Mexico;2. Departamento de Nanotecnología, Centro de Física Aplica y Tecnología Avanzada, Universidad Nacional Autónoma de México, A.P. 1-1010, Querétaro, Qro. C.P. 7600, Mexico;3. Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, A.P. 1-1010, Edo. de México, Mexico;4. Posgrado en Ciencias Químico-Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Las Campanas, C.P. 76010 Santiago de Querétaro, Qro., Mexico;1. Department of Chemical Sciences, University of Naples Federico II, Via Cintia 4, I-80126 Napoli, Italy;2. Department of Biology, University of Naples Federico II, Via Cintia 4, I-80126 Napoli, Italy;3. Department of Experimental Medicine, Second University of Naples, via de Crecchio 7, I-80138 Napoli, Italy;4. Bionap srl, R&D Contrada Fureria, ZIO 950125 Piano Tavola, Belapsso, Italy;5. Department of Drug Sciences, University of Catania, Catania, Italy;1. Natural Products Analysis Laboratory, Faculty of Science, Taif University, Saudi Arabia;2. Medicinal Chemistry Laboratory, Theodor Bilharz Research Institute, Giza, Egypt;3. Plant Production Department, Faculty of Environmental Agricultural Sciences, Suez Canal University, El Arish, Egypt;1. Department of Biotechnology and Food Technology, Durban University of Technology, P.O. Box 1334, Durban, 4000, South Africa;2. Agricultural Research Council, Roodeplaat Vegetable and Ornamental Plants, Private Bag X293, Pretoria, 001, South Africa;1. Analytical Department, MC Analysis Center ltd, 2563 Nicosia, Cyprus;2. Laboratory of Food Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece;3. Institute of Sciences of Food Production (ISPA), CNR, 73100 Lecce, Italy |
| |
| Abstract: | Cladode ultrastructural features of two prickly and two spineless Opuntia ficus-indica cultivars were examined using environmental scanning electron and atomic force microscopies. Observations focused on cladode as well as spine and glochid surface micromorphologies. Prickly cultivars were characterized by abundant cracked epicuticular wax deposits covering the cladode surface, with an amorphous structure as observed by AFM, while less abundant waxy plates were observed by ESEM on spineless cultivar cladodes. Further AFM observations allowed a rough granular and crystalloid epicuticular wax structure to be distinguished in spineless cultivars. Regarding spine micromorphology, prickly cultivars had strong persistent spines, observed by ESEM as a compact arrangement of oblong epidermal cells with a rough granular structure. However, deciduous spines in spineless cultivars had a broken transversely fissured epidermis covering a parallel arrangement of fibres. Through AFM, the deciduous spine surface presented an irregular hilly and smooth microrelief while persistent spines exhibited rough helical filamentous prints.ESEM and AFM studies of cladode surfaces from prickly and spineless cactus pear cultivars revealed valuable micro-morphological details that ought to be extended to a large number of O. ficus-indica cultivars. |
| |
| Keywords: | Cactus pear Ultrastructure Spine ESEM AFM |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
