Examination of the transition of cultured neuronal cells from submerged to exposed using an environmental scanning electron microscope (ESEM)期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Examination of the transition of cultured neuronal cells from submerged to exposed using an environmental scanning electron microscope (ESEM)

Affiliation:	1. PEPITE EA4267, FHU INCREASE, Univ. Bourgogne Franche-Comté, F-25030 Besançon, France;2. INSERM UMR 1093-CAPS, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France;1. Department of Surgical, Medical, Dental and Morphological Sciences with Interest in Transplants, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy;2. EURAC Research, Center for Biomedicine, Bolzano, Italy;3. Unit of Obstetrics and Ginecology, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy;1. Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy;2. Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy;3. Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy

Abstract:	Relatively few studies of fully hydrated live or fixed cultured animal cells viewed by environmental scanning electron microscopy (ESEM) have been published. In some cases there may have been some drying out of the cells. In this study the interface between water and cells is imaged as water is carefully evaporated to expose cells. Technical difficulties associated with the process, including inadvertent rewetting of cells are described. Suggestions are made for optimising operating parameters for viewing fully hydrated cultured cells by ESEM. The prospects for viewing live cultured cells are discussed.

Keywords:	Fully hydrated Biological specimens Neuronal processes Cellular protrusions
本文献已被 ScienceDirect 等数据库收录！

设为首页 | 免责声明 | 关于勤云 | 加入收藏