Mechanisms of photosensitized DNA cleavage |
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Authors: | Nicole Paillous Patricia Vicendo |
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Institution: | Laboratoire des IMRCP, URA 470 au CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex France |
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Abstract: | Photosensitization may promote DNA damages such as nucleic acid oxidation or single strand breaks via three main pathways: hydroxyl radicals attack, electron transfer process or oxidation by singlet oxygen. While direct production of OH. by photosensitization is rarely observed, the mechanism of DNA attack by OH. is now well established on the basis of informations provided by water radiolysis experiments. Some dyes may also induce single strand breaks via an electron transfer occurring from a nucleobase to the sensitizer in the excited state. This process generates base radical cations identical to those arising from DNA photoionisation. These radicals may undergo deprotonation or dehydration to form the same neutral radicals as those produced by OH. but with a slightly different pattern. In contrast, while many sensitizers produce singlet oxygen, the mechanism of DNA damages induced by this way is still unclear. In this case the guanine moiety in nucleosides or in DNA is selectively altered leading to the formation of 8 oxoG or 8 oxodG and FapyGua. The mechanism of single strand breaks formation by singlet oxygen is discussed in this overview. |
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Keywords: | Hydroxyl radicals Singlet oxygen Electron transfer DNA damage 8-oxoguanine FapyGua |
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