Synthesis and incorporation into DNA of a chemically stable, functional abasic site analogue |
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Authors: | Mosimann Markus Küpfer Pascal A Leumann Christian J |
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Institution: | Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland. |
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Abstract: | reaction: see text] The abasic site building block 7 for DNA synthesis, containing a methylenephosphinic acid group at C3', was prepared in six steps and was incorporated into DNA via a combination of H-phosphonate and phosphoramidite chemistry. Corresponding oligodeoxynucleotides were shown to be chemically stable under basic conditions and fully functional at the respective hemiacetal center. |
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