Sample-induced internal pH gradients in microcolumn liquid chromatography of proteins |
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| Authors: | M. Janeček J. Šalamoun K. Šlais |
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| Affiliation: | (1) Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Veve!["rcaron"]("/content/yq1722854v82763r/xlarge345.gif") í 97, 611 42 Brno, Czechoslovakia |
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| Abstract: | Summary The technique of an internal pH gradient induced by the sample is applied to the separation of proteins by liquid chromatography. Compatibility of the method with microcolumns is demonstrated and examples of separations on different types of sorbents are given. |
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| Keywords: | Column liquid chromatography Sample-induced pH gradients Separation of proteins |
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