Purification of Phleum pratense pollen extract by immunoaffinity chromatography and high-performance ion-exchange chromatography |
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| Authors: | E Bolzacchini P Di Gennaro G Di Gregorio B Rindone P Falagiani G Mistrello I Sondergaard |
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| Institution: | Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy. |
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| Abstract: | Basic allergens of Phleum pratense pollen extract have been purified by either sequence gel filtration-ion-exchange high-performance liquid chromatography (HPIEX) and size-exclusion HPLC or sequence gel filtration-immunoaffinity chromatography and HPIEX. The second procedure seems to be suitable for preparative purposes. |
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