Application of capillary electrophoresis to the analysis of soluble chromatin |
| |
| Authors: | Asatiani N V Abuladze M K Birkaya B G Sapojnikova N A Tsibakhashvili N Y Mosulishvili L M |
| |
| Institution: | Institute of Physics, Georgian Academy of Sciences, Tamarashvili str. 6, Tbilisi, Georgia. |
| |
| Abstract: | Capillary electrophoresis (CE) has been applied to study DNA-protein complexes using as the test system soluble chromatin from chicken erythrocytes and rapidly proliferated cultured Chinese hamster fibroblast-like cells B11-dii-FAF-28. Separation was performed with home-made CE apparatus, using a regulated high-voltage power supply, UV-detector and fused silica capillaries with inner diameter 75 microm. The heterogeneity of nucleosomal particles with different DNA lengths after micrococcal nuclease digestion was detected. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
