| D-苯甘氨酸手性固定相的制备及对多种手性化合物的拆分 |
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| 引用本文： | 常银霞,周玲玲,向兰,任朝兴,袁黎明. D-苯甘氨酸手性固定相的制备及对多种手性化合物的拆分[J]. 分析测试学报, 2007, 26(1): 107-109 |
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| 作者姓名： | 常银霞 周玲玲 向兰 任朝兴 袁黎明 |
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| 作者单位： | 云南师范大学,化学化工学院,云南,昆明,650092;山西师范大学,化学与材料科学学院,山西,临汾,041000;云南师范大学,化学化工学院,云南,昆明,650092;昆明市生产力促进中心,云南,昆明,650000 |
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| 基金项目： | 高等学校优秀青年教师教学科研奖励计划项目(2001298)，云南省自然科学基金资助项目(2004B0013R) |
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| 摘 要： | 以D-3,5-二硝基苯甲酰苯甘氨酸和3-氨丙基硅烷化硅胶合成了D-苯甘氨酸衍生手性固定相,并自制了手性高效液相色谱分离柱,用正己烷-异丙醇作流动相,对非衍生化的氨基酸、胺、醇和羧酸类等10种手性化合物进行了高效液相色谱拆分。结果表明:所拆分的10种手性化合物,有7种手性化合物能得到基线分离,最好的分离度Rs=5.56。该文还用苯对柱性能进行了评价,理论塔板数达每米8万块。
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| 关 键 词： | D-苯甘氨酸衍生手性固定相 高效液相色谱 手性拆分 |
| 文章编号： | 1004-4957（2007）01-0107-03 |
| 收稿时间： | 2005-11-12 |
| 修稿时间： | 2006-03-27 |
Separation of Various Racemates Using D-Phenylglycine as Chiral Stationary Phase |
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| CHANG Yin-xia,ZHOU Ling-ling,XIANG Lan,REN Chao-xing,YUAN Li-ming. Separation of Various Racemates Using D-Phenylglycine as Chiral Stationary Phase[J]. Journal of Instrumental Analysis, 2007, 26(1): 107-109 |
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| Authors: | CHANG Yin-xia ZHOU Ling-ling XIANG Lan REN Chao-xing YUAN Li-ming |
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| Affiliation: | 1. School of Chemistry. and Chemical Science, Yunnan Normal University, Kunming 650092, China ; 2. School of Chemistry and Material Science, Shanxi Normal University, Linfen 041000, China; 3. Kunming Productivity Promotion Center, Kunming 650000, China |
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| Abstract: | D-Phenylglycine derivative chiral stationary phase(CSP) was synthesized with D-3,5dinitrobenzoylphenylglycine and 3-aminopropyl silica gel and the chiral column was prepared.Ten nonderivatives racemates,which were amino acids,amines,alcohols and carboxylic acids,were separated on the CSP using hexane and isopropanol as mobile phase by high-performance liquid chromatography(HPLC). The results showed that seven of the ten enantiomers could be baseline separated and the best value of resolution factors(Rs) was 5.56.The evaluation of column efficiency was carried out by benzene and the number of theoretical plate was 80 000 /m. |
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| Keywords: | D-Phenylglycine derivative CSP HPLC Chiral separation |
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