| 测定抗坏血酸的亚甲蓝褪色光度法 |
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| 引用本文： | 陈焕文,宋庆宇,刘宏伟,金钦汉.测定抗坏血酸的亚甲蓝褪色光度法[J].分析测试学报,2001,20(2):55-57. |
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| 作者姓名： | 陈焕文 宋庆宇 刘宏伟 金钦汉 |
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| 作者单位： | 长春分析仪器研究和技术开发中心吉林大学化学系, |
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| 摘 要： | 确定了亚甲蓝与抗坏血酸褪色反应的最佳条件，在 pH=2的缓冲溶液和对氨基苯磺酸存在的条件下，抗坏血酸能够很快还原亚甲蓝并使之褪色，褪色反应的体系具有很好的稳定性。该反应的摩尔吸光系数为 2.0× 103 L· mol－ 1· cm－ 1，线性范围 0.4～ 40.0 mg/L。该法具有优良的选择性，常见的能够与抗坏血酸共存的阴、阳离子和还原剂、表面活性剂不干扰测定，应用于药品、饮料、蔬菜中抗坏血酸的测定，获得满意结果。
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| 关 键 词： | 抗坏血酸 亚甲蓝 褪色光度法 维生素C 药品 饮料 蔬菜 测定 |
| 文章编号： | 1004-4957(2001)02-0055-03 |
| 修稿时间： | 2000年6月30日 |
Determination of Ascorbic Acid by Spectrophotometry with Fading Reaction of Methylene Blue |
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| CHEN Huan-wen,SONG Qing-yu,LIU Hong-wei,JIN Qin-han.Determination of Ascorbic Acid by Spectrophotometry with Fading Reaction of Methylene Blue[J].Journal of Instrumental Analysis,2001,20(2):55-57. |
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| Authors: | CHEN Huan-wen SONG Qing-yu LIU Hong-wei JIN Qin-han |
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| Abstract: | Methylene blue is reduced and faded by ascorbic acid in the medium of dilute hydrochloric acid（ pH=2） with sulfanilic acid as a catalyst.The optimal conditions of the fading reaction were investigated in detail.Based on this fading reaction a new spectrophotometric method for the determination of ascorbic acid was established.The apparent molar absorptivity of the system was ε 660=2.0× 103 L· mol－ 1· cm－ 1,whereas the linear range was 0.4～ 40.0 mg/L for ascorbic acid.The method is rapid,sensitive and selective.Common coexisting ions,reducing agents and surfactant do not interfere with the determination.This method was used to determination of ascorbic acid in pharmaceuticals,soft drinks and vegetables with satisfactory results. |
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| Keywords: | Ascorbic acid Methylene blue Fading reaction |
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