肌动蛋白与顺铂、反铂的相互作用

利用1H-NMR研究了肌动蛋白(actin)与顺铂、反铂的相互作用，测定了肌动蛋白的聚合临界浓度，并研究了顺伯、反铂对它的影响。实验结果显示顺伯和反驳都能导致微丝（F-actin）解聚。在相同浓度下，反铂的作用更加明显。对于肌动蛋白单体(G-actin)，顺铂在低浓度下促进其聚合。反铂则只表现出抑制G-actin的聚合。讨论了顺铂、反铂与G-actin可能的结合位点，顺铂可能与Cys-10和Cys-285结合，而反铂不与Cys-10结合。

Interaction between Actin and Cisplatin or Transplatin

The interaction between actin and cisplatin or transplatin has been studied by using the 1H- NMR spectra and measuring the critical concentration of actin polymerization.These studies show that both cisplatin and transplatin induce a depolymerization of F- actin,and that the effect of transplatin is more obvious than that of cisplatin at the same concentration.It is found that cisplatin promotes a polymerization of G- actin at low concentration and inhibits it at high concentration,while transplatin inhibits the polymerization at any concentration.The binding sites of cisplatin / transplatin to G- actin are discussed.It is probable that cisplatin binds with Cys- 10 and Cys- 285,while transplatin does not bind with Cys- 10.