Liquid chromatography-diode array detection-electrospray ionisation mass spectrometry/nuclear magnetic resonance analyses of the anti-hyperglycemic flavonoid extract of Genista tenera. Structure elucidation of a flavonoid-C-glycoside |
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Authors: | Rauter Amélia P Martins Alice Borges Carlos Ferreira Joana Jorge Justino Bronze Maria-Rosário Coelho Ana V Choi Young H Verpoorte Robert |
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Institution: | Departamento de Química e Bioquímica/Centro de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Ed C8, 5 Piso, 1749-016 Lisboa, Portugal. aprauter@fc.ul.pt |
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Abstract: | The anti-hyperglycemic flavonoid extract obtained from Genista tenera was first studied by liquid chromatography (LC)-diode array detection (DAD) which showed the presence of two major compounds. One of them was identified as genistein-7-O-glucoside. Luteolin-7-O-glucoside was detected as a minor constituent, while luteolin-7,3'-di-O-glucoside and rutin were found in trace amounts. LC-DAD-ESI-MS and NMR were used to confirm the structure of these compounds and allowed the elucidation of the structure of the unknown major compound, which is the flavonoid 5,7,4'-trihydroxyisoflavone-8-C-glucoside. |
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