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Use of a preparative-scale, recirculating isoelectric trapping device for the isolation and enrichment of acidic proteins in bovine serum
Authors:Shave Evan  Vigh Gyula
Institution:Department of Chemistry, Texas A&M University, College Station, TX 77842-3012, USA.
Abstract:A recirculating, preparative-scale isoelectric trapping device, developed for the binary isoelectric trapping separation of proteins has been used to desalt, isolate and enrich the pI<4 protein fraction from a 150 mL sample of bovine serum. Subsequent re-separation of the 2
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