| 利用ITS序列对两个盐藻株的分子鉴定 |
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| 引用本文： | 王伟,许政暟,宋任涛.利用ITS序列对两个盐藻株的分子鉴定[J].上海大学学报(自然科学版),2006,12(1):84-88. |
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| 作者姓名： | 王伟 许政暟 宋任涛 |
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| 作者单位： | 1. 上海大学,生命科学学院,上海,200444
2. 上海大学,生命科学学院,上海,200444;中科院,上海植物生理生态研究所,上海,200032 |
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| 摘 要： | 对实验室长期保存的两个盐藻株(Dunaliella salina)核糖体rDNA的ITS(internal transcribed space)序列(ITS1 5.8S rDNA ITS2)用PCR技术扩增并克隆,经序列测定后,与从GenBank中获得的相关序列一起构建系统发育树.结果显示:其中一个(Dunaliella salinaSHU 01)与Dunaliella viridisCONC 002的距离最近,另一个(Dunaliella salinaSHU02)与Dunaliella salinaUTEX 1644的距离最近.因此分别命名为Dunaliella viridisSHU与Dunaliella salinaSHU.
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| 关 键 词： | 盐藻 ITS序列 系统发育树 |
| 文章编号： | 1007-2861（2006）01-0084-05 |
| 收稿时间： | 2005-01-24 |
| 修稿时间： | 2005年1月24日 |
Identification of Two Dunaliella sp. Based on Nuclear ITS rDNA Sequences |
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| WANG Wei,XU Zheng-kai,SONG Ren-tao.Identification of Two Dunaliella sp. Based on Nuclear ITS rDNA Sequences[J].Journal of Shanghai University(Natural Science),2006,12(1):84-88. |
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| Authors: | WANG Wei XU Zheng-kai SONG Ren-tao |
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| Institution: | 1. School of Life Sciences, Shanghai University, Shanghai 200444, China 2. Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China |
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| Abstract: | The internal transcribed spaces(ITS) of the two Dunaliella sp.(Dunaliella salina) were cloned by PCR and sequenced.An ITS phylogenetic tree was constructed based on combination of sequence data set from GenBank.Based on this "tree",one(Dunaliella salina SHU 01) was mostly close to Dunaliella viridis CONC 002 and the other(Dunaliella salina SHU 02) was close to Dunaliella salina UTEX 1644.Therefore,Dunaliella salina SHU 01 is named as Dunaliella viridis SHU,and Dunaliella salina SHU 02 as Dunaliella salina SHU. |
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| Keywords: | Dunaliella internal transcribed space phylogenetic tree |
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