环糊精聚合物的分子包合作用及在酶电极中的应用

伏安法用于研究环糊精预聚合物的分子包合作用．红外光谱实验表明环糊精预聚合物与戊二醛缩聚生成的聚合物带有悬挂的羰基，后者能使葡萄糖氧化酶共价固定化．由于分子包合作用，电子受体可存储在含酶的环糊精聚合物膜中，从而提高了酶膜中电子受体的浓度又减少了电子受体的用量．用ＴＴＦ等作电子受体，可实现酶和电子受体在环糊精聚合物中的同时固定化．环糊精聚合物膜中的组成和膜厚度可以控制，为酶电极的基础研究工作提供了方便．

Molecular Inclusion of Cyclodextrin Polymer and Its Application to Enzyme Electrodes

The inclusion complexation of α-cyclodextin prepolymer host with benzoquinone guest was studied by voltametry. The spectroscopic measurements show that the polycondensation of CD with glutaric dialdehyde forms the polymer (PCD) with pending carbonyl, the latter brings about covalent immobilisation of glucose oxidase (GOD) to polymer chains. Owing to the inclusion complexation of CD, the benzoquinone(BQ) as mediator of GOD can be collected in the enzyme containing PCD membrane, resulting in high concentration of BQ in the membrane while decreasing its amount in solution. TTF was tested to prepare the immobilised enzyme together with mediator in PCD. The enzyme loading in PCD membrane and the membrane thickness can be controlled easily, which will facilate to fundmental reshearch of enzyme electrodes.