| 茄青枯假单胞菌T2015致病相关基因突变体的构建 |
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| 引用本文： | 赵昕梅,冯家勋.茄青枯假单胞菌T2015致病相关基因突变体的构建[J].信阳师范学院学报(自然科学版),2005,18(4):413-415. |
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| 作者姓名： | 赵昕梅 冯家勋 |
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| 作者单位： | 1. 信阳师范学院,生命科学学院,河南,信阳,464000
2. 广西大学,生命科学与技术学院,广西,南宁,530005 |
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| 基金项目： | 国家自然科学基金资助项目(39970011) |
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| 摘 要： | 分离自花生植株的茄青枯假单胞菌(Ralstonia solanacearum)T2015的Tn5-lacZ插入突变体T2135是极性突变体.采用现代分子生物学实验技术,首先构建了同一操纵子中ORF2的表达质粒pGX6191,并进行生物学验证,然后采用三亲本结合技术导入极性突变体T2135,得到茄青枯假单胞菌T2015中与致病相关基因ORF3的突变体T2135/R.
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| 关 键 词： | 茄青枯假单胞菌 花生 操纵子 毒性基因 |
| 文章编号： | 1003-0972（2005）04-0413-03 |
| 收稿时间： | 2004-10-26 |
| 修稿时间： | 2004-10-26 |
Construction of the pathogenic gene's mutant of Rs. T2015 |
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| ZHAO Xin-mei,FENG Jia-xun.Construction of the pathogenic gene''''s mutant of Rs. T2015[J].Journal of Xinyang Teachers College(Natural Science Edition),2005,18(4):413-415. |
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| Authors: | ZHAO Xin-mei FENG Jia-xun |
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| Institution: | 1. College of Life Sciences, Xinyang Normal University, Xinyang 464000, China ; 2. Dept. of Biol. and Tec. ,Guangxi University ,Nanning 530005 ,China |
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| Abstract: | The mutant T2135 inserted by Tn5-lacZ is a polar mutant of Ralstonia solanacearum T2015 isolated from peanut. Modern molecular biology experimental technique was used to construct the expression plasmid pGX6191 of ORF2 at first and biological proof was carried out, then the expression plasmid pGX6191 was mated into the polar mutant T2135 by tri-parent,and pathogenic relevant gene ORF3's mutant T2135/R was obtained. |
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| Keywords: | Ralstonia solanacearum peanut operon virulence gene |
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