Determination of chloramphenicol in milk by a fluorescence polarization immunoassay |
| |
| Authors: | N V Gasilova S A Eremin |
| |
| Institution: | (1) The Institute of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, China;(2) Institute of Agri-Food and Land Use, Queen’s University Belfast, Belfast, BT9 5AG, UK;(3) Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119998, Russia; |
| |
| Abstract: | A procedure for the determination of the drug chloramphenicol using a fluorescence polarization immunoassay (FPIA) was proposed.
The optimum pairs of antibodies and antigens labeled with fluorescein were chosen, and the analytical characteristics of the
procedure were determined. A rapid procedure for milk sample preparation with the use of a saturated solution of ammonium
sulfate was optimized. The total time of sample preparation and determination of chloramphenicol in milk was no longer than
10 min. The detection limits of chloramphenicol in water and milk were 10 ng/mL and 20 μg/kg, respectively. The procedure
developed for the determination of chloramphenicol was tested in the analysis of model and real milk samples. It was found
that some milk samples contained chloramphenicol in concentrations of 38–41 μg/kg, which are several times higher than the
maximum permissible concentration (MPC) (10 μg/kg). |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
