A catalytically active molecularly imprinted polymer that mimics peroxidase based on hemin: application to the determination of<Emphasis Type="Italic"> p</Emphasis>-aminophenol |
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Authors: | Wilney de Jesus Rodrigues Santos Phabyanno Rodrigues Lima César Ricardo Teixeira Tarley Lauro Tatsuo Kubota |
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Institution: | (1) Departamento de Química Analítica, Instituto de Química, Universidade Estadual de Campinas, Campinas, SP 13084–971, Brazil;(2) Departamento de Ciências Exatas, Universidade Federal de Alfenas, Alfenas, MG 37130–000, Alfenas-MG, Brazil |
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Abstract: | Despite the increasing number of applications of molecularly imprinted polymers (MIP) in analytical chemistry, the synthesis
of polymers with hemin introduced as the catalytic center to mimic the active site of peroxidase remains as a challenge. In
the current work, a new type of molecularly imprinted polymer (MIP) was synthesized with 4-aminophenol (4-APh) as the template
and two monomers: hemin, which acts as the catalytic center, and methacrylic acid (MAA), which is used to build the active
sites. This work shows that MIP successfully mimics peroxidase. For this purpose, a flow injection analysis system coupled
to an amperometric detector was investigated through multivariate analysis. The determination of 4-APh was not affected by
the equimolar presence of structurally similar phenol compounds, including catechol, 4-chloro-3-methylphenol, 2-aminophenol,
guaiachol, chloroguaiachol and 2-cresol, thus highlighting the good performance of the imprinted polymer. Under the optimized
experimental conditions, an analytical curve covering a wide linear response range from 0.8 up to 500 μmol L−1 (r > 0.999) was obtained, and the method gave satisfactory precisions (n = 8), as evaluated via the relative standard deviation (RSD), of 4.1 and 3.2% for solutions of 4-APh of 50 and 500 μmol L−1, respectively. Recoveries of 96–111% from water samples (tap water and river water) spiked with 4-APh were achieved, thus
illustrating the accuracy of the proposed system.
Figure Schematic presentation of the synthesis of the MIP |
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Keywords: | 4-Aminophenol Hemin Molecularly imprinted polymers Amperometric determination |
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