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| CdTe/ZnSe核壳量子点免疫层析试纸条检测克伦特罗的研究 | |
| 引用本文： | 胡华军,付涛,张明洲,洪治,陈宗伦,刘军. CdTe/ZnSe核壳量子点免疫层析试纸条检测克伦特罗的研究[J]. 分析化学, 2010, 38(12). DOI: 10.3724/SP.J.1096.2010.01727 |
| 作者姓名： | 胡华军 付涛 张明洲 洪治 陈宗伦 刘军 |
| 作者单位： | 1. 中国计量学院生命科学学院,杭州,310018;浙江省生物计量及检验检疫技术重点实验室,杭州,310018 2. 中国科学院苏州纳米技术与纳米仿生研究所,苏州,215125 3. 中国计量学院太赫兹技术与应用研究所,杭州,310018 4. 中国计量学院生命科学学院,杭州,310018 |
| 基金项目： | 浙江省自然科学基金(No.Y3080390)资助 |
| 摘 要： | 采用巯基丁二酸作为表面修饰剂,水相法合成水溶性的CdTe/ZnSe核壳量子点,然后在N-羟基琥珀酰亚胺(NHS)的作用下,将CdTe/ZnSe核壳量子点与抗克伦特罗多克隆抗体(Anti-CLE pAb)连接。通过凝胶电泳和斑点杂交实验,验证CdTe/ZnSe核壳量子点与Anti-CLE pAb连接成功,并且CdTe/ZnSe-Anti-CLE pAb偶联物能识别克伦特罗-BSA抗原(CLE-BSA)。光谱分析表明,量子点与抗体连接后荧光增强,荧光峰位从628nm红移至635nm。将合成的CdTe/ZnSe-Anti-CLE pAb偶联物作为指示克伦特罗(CLE)分子的荧光标记物,制备出一种用于检测CLE的免疫层析试纸条,其最低检测量可达1μg/L。与ELISA法的对比实验表明,此试纸条能应用于CLE残留的快速检测。 |
| 关 键 词： | CdTe/ZnSe核壳量子点 克伦特罗 免疫层析 |
Development of CdTe/ZnSe Core/Shell Quantum Dots-based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol |
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| HU Hua-Jun,FU Tao,ZHANG Ming-Zhou,HONG Zhi,CHEN Zhong-Lun,LIU Jun. Development of CdTe/ZnSe Core/Shell Quantum Dots-based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol[J]. Chinese Journal of Analytical Chemistry, 2010, 38(12). DOI: 10.3724/SP.J.1096.2010.01727 | |
| Authors: | HU Hua-Jun FU Tao ZHANG Ming-Zhou HONG Zhi CHEN Zhong-Lun LIU Jun |
| Affiliation: | HU Hua-Jun1,2,FU Tao3,ZHANG Ming-Zhou1,HONG Zhi4,CHEN Zhong-Lun1,LIU Jun 1,2 1(College of Life Science,China Jiliang University,Hangzhou 310018) 2(Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine,Hangzhou 310018) 3(Suzhou Institute of Nano-tech and Nano-bionics,Chinese Academy of Sciences,Suzhou 215125) 4(Centre for THz Research,Hangzhou 310018) |
| Abstract: | CdTe/ZnSe core/shell quantum dots(QDs) were synthesized in aqueous solution by using mercaptosuccinic acid as the stabilizer,and then were coupled to the polyclonal antibody(Anti-Clenbuterol pAb,Anti-CLE pAb) with N-hydroxysuccinimide(NHS).The coupled product(CdTe/ZnSe-Anti-CLE pAb) was confirmed by agarose gel electrophoresis method,and its antibody activity was tested by protein dot-blot assay.Spectra analysis further showed that the photoluminescence(PL) intensity of the CdTe/ZnSe-Anti-CLE pAb was enhanc... |
| Keywords: | Cadmium telluride/zinc selenide core/shell quantum dots Clenbuterol Immunochromatography |
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