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| USE OF HUMAN ERYTHROCYTE GHOSTS FOR TRANSFER OF~(125)I-BSA AND~(125)I-DNA INTO ANIMAL CELLS THROUGH CELL FUSION | |
| 作者姓名： | 李昌本 赵寿元 |
| 作者单位： | Institute of Genetics，Fudan University，Shanghai，Institute of Genetics，Fudan University，Shanghai |
| 摘 要： | ~(125)I-BSA and human ~(125)I-DNA fragments have been prepared for tests of loading efficiencyby the erythrocyte ghosts, which is found to be 3.7 and 3.0 per cent respectively. The experi-ments are reproduceable. The ~(125)I-DNA fragments trapped in the erythrocyte ghosts aretransferred into DON cells through cell fusion or "microrinjection". The injection efficiencyappraised by autoradiography amounts to 58 per cent. |
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