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| 壳聚糖膜固定生物活性短肽RGDS的研究 | |
| 引用本文： | 赵晓飞,王迎军,卢玲,任力,陈晓峰,葛坚.壳聚糖膜固定生物活性短肽RGDS的研究[J].化学研究与应用,2006,18(12):1369-1374. |
| 作者姓名： | 赵晓飞 王迎军 卢玲 任力 陈晓峰 葛坚 |
| 作者单位： | 1. 华南理工大学材料学院生物材料研究所,广东,广州,510640 2. 华南理工大学材料学院生物材料研究所,广东,广州,510640;特种功能材料及其制备新技术教育部重点实验室,华南理工大学,广东,广州,510640 3. 中山眼科中心,中山大学,广东,广州,510060 |
| 基金项目： | 国家“863”项目(2003AA205053)资助，国家“973”项目(2005CB623902)资助，国家自然科学基金项目(50472054)资助 |
| 摘 要： | 为改善壳聚糖对细胞的特异性吸附,采用水溶性碳二亚胺将生物活性短肽精氨酸-甘氨酸-天冬氨酸-丝氨酸(RGDS)固定到壳聚糖膜的表面,采用X射线光电子能谱检测固定多肽前后的壳聚糖膜表面,发现反应后壳聚糖膜表面氮元素含量增大,Nls和Cls曲线拟合谱中酰胺键增多,表明RGDS短肽已固定到壳聚糖膜的表面;人角膜缘上皮细胞体外培养实验表明,固定RGDS后壳聚糖膜的细胞黏附率有了明显提高,固定RGDS后的壳聚糖膜在角膜组织工程支架等方面有更好的应用潜力。 |
| 关 键 词： | 壳聚糖 RGDS XPS 细胞黏附率 组织工程支架 |
| 文章编号： | 1004-1656（2006）12-1369-06 |
| 收稿时间： | 2006-03-20 |
| 修稿时间： | 2006-08-18 |
Study on RGDS-immobilized chitosan films |
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| ZHAO Xiao-fei,WANG Ying-jun,LU Ling,RE Li,CHEN Xiao-feng,GE Jian.Study on RGDS-immobilized chitosan films[J].Chemical Research and Application,2006,18(12):1369-1374. | |
| Authors: | ZHAO Xiao-fei WANG Ying-jun LU Ling RE Li CHEN Xiao-feng GE Jian |
| Institution: | 1. Biomaterials research institute, College of Materials Science and Engineering, South China University of Technology, Guangdong 510640 , China; 2. Key Laboratory of Specially Functional Materials and Advanced Manufacturing Technology of Ministry of Education, South China University of Technology, Guangdong 510640 , China ; 3. ZhongShan Ophlthalmie Center, Sun Yat-sen University, Guangdong 510060,China |
| Abstract: | Chitosan films were modified with RGDS(Arg-Gly-Asp-Ser)Via an imide-bond forming reaction between amino groups in chitosan and carboxyl groups in peptides.The surface characterization was carried out by X-ray photoelectron spectroscopy(XPS).The results showed that the composition fraction of nitrogen(Nls)for chitosan and that for chitosan-RGDS surface are 6.64%and 10.87%,respectively.It was confirmed that RGDS was successfully immobilized onto chitosan surfaces.Human corneal epithelium(HCE)cells were cultured onto the CS and CS-RGDS films respectively.The MTT results showed that the cell density onto the RGDS-modified films is higher than on the unmodified CS film.It was demonstrated that RGDS immobilization onto the chitosan could improve the attachment of HCE cells.RGDS-immobilized chitosan can be a promising candidate material for corneal tissue engineering. |
| Keywords: | chitosan RGDS XPS cell adhersion rate tissue engineering scaffold |
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