Detection of nerve agent markers by liquid chromatography-mass spectrometry |
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| Authors: | I. A. Rodin A. V. Braun I. A. Anan’eva O. A. Shpigun E. I. Savel’eva I. V. Rybal’chenko S. L. Bolotov G. M. Rodchenkov |
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| Affiliation: | 1.Department of Chemistry,Moscow State University,Moscow,Russia;2.Scientific-Research Institute of Hygiene, Occupational Pathology, and Human Ecology,Russian Ministry of Health,Vsevolzhskii region, Leningrad oblast,Russia;3.Lyumekszashchita Research Production Company,Moscow,Russia;4.Anti Doping Center,Ministry of Sport, Tourism, and Youth Policy,Moscow,Russia |
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| Abstract: | An approach to the detection of metabolites of organophosphorous agents (OPA), such as O-iso-propylmethylphosphonic acid (detection limit, 4 ng/mL), O-pinacolylmethylphosphonic acid (0.6 ng/mL), and O-isobutylmethylphosphonic acid (1 ng/mL), in plasma samples was developed using liquid chromatography-mass spectrometry. The curves of the elutionxcretion of OPA metabolites were obtained for the samples of biological material of rats exposed to toxic substances. Determination was performed by mass spectrometry with electrospray ionization in the negative ion mode, using deprotonated molecules for detection. The biological samples were analyzed by reversed-phase chromatography using hydrophilic end-capped adsorbents. Solid phase extraction on reversed-phase adsorption cartridges containing a copolymer of styrene and divinylbenzene was proposed for sample preparation. |
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