Rapid chemical synthesis of d(CACGTG) in milligram amounts by solution-phase phosphotriester chemistry |
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| Authors: | G V Rajendrakumar N Sivakamisundari K N Ganesh |
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| Affiliation: | (1) Centre for Cellular and Molecular Biology, 500 007 Hyderabad, India;(2) Present address: Division of Organic Chemistry, National Chemical Laboratory, 411 008 Pune, India |
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| Abstract: | This paper reports an optimised method for chemical synthesis of short oligodeoxyribonucleotides in milligram amounts by solution-phase phosphotriester chemistry. The procedure is a slight modification of one reported earlier in the literature. Detailed experimental procedures for condensation, deprotection and purification are described. The efficiency of the method is demonstrated by the synthesis of a hexamer, d(CACGTG), in good yields and high purity. The homogeneity of the product and the sequence composition are shown by 270 MHz1H NMR spectrum. |
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| Keywords: | Oligodeoxyribonucleotide synthesis chemical synthesis of d(CACGTG) 1H NMR of oligonucleotides |
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