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Synthesis and toxicity assay of ceramic nanophosphors for bioimaging with near-infrared excitation
Authors:Nallusamy Venkatachalam  Eva Hemmer  Tomoyoshi Yamano  Hiroshi Hyodo  Hidehiro Kishimoto  Kohei Soga
Institution:1. Polyscale Technology Research Center, Tokyo University of Science, Japan;2. Center for Technologies against Cancer, Tokyo University of Science, Japan;3. Division of Immunobiology, Research Institute for Biological Science, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan;4. Department of Materials Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan;1. Department of Applied Physics and Chemistry, The University of Electro-Communications, Tokyo 182-8585, Japan;2. Department of Engineering Science, The University of Electro-Communications, Tokyo 182-8585, Japan;1. School of Chemistry, Monash University, Clayton, Victoria 3800, Australia;2. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, PR China;1. School of Chemical Engineering, Northwest University, Xi’an 710069, China;2. Department of Physics, Northwest University, Xi’an 710069, China;1. State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China;2. University of the Chinese Academy of Sciences, Beijing 100049, PR China;1. College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, 10520, Thailand;2. Nanotec-KMITL Center of Excellence on Nanoelectronics Device, Bangkok, 10520, Thailand;3. Thailand Center of Excellence in Physics, CHE, Bangkok, 10400, Thailand;1. College of Chemistry, Nanchang University, Nanchang 330031, China;2. College of Materials Science and Engineering, Nanchang University, Nanchang 330031, China
Abstract:Yttrium hydroxyl carbonate (Y(OH)CO3) precursors were synthesized by the homogeneous co-precipitation method in the presence of polyacrylic acid (PAAc). Resultant precursor particle size is about 15–20 nm with narrow size distribution whereas the particle size is smaller than those acquired by the conventional homogeneous precipitation method. Effective decrease of Y(OH)CO3 particle size was found to be higher for the presence of weak polyanionic ionomer such as PAAc than the presence of strong polyanionic ionomer such as sodium polystyrene sulfonate (PSS). It was observed that the morphology and size of the precursors are almost unchanged after the calcination process. Er3+ doped Y2O3 nanoparticles were synthesized by PAAc assisted homogeneous co-precipitation method showed bright green (550 nm) and red (660 nm) upconversion (UC) as well as near-infrared (NIR) fluorescence (1550 nm) under 980-nm excitation. UC and NIR fluorescence bioimaging and in-vitro cytotoxicity assay of Er3+ doped Y2O3 nanoparticles were successfully attempted with commercially available macrophages and B-cell hybridomas. Cellular uptake of nanoparticles is evidenced from bright field, UC and NIR fluorescence images of macrophages.
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